US 8,617,573 B2 · Botulinum neurotoxin B receptors and use thereof

Abstract

It is disclosed here that synaptotagmin I (syt I) and synaptotagmin II (syt II) are the cellular receptors for botulinum neurotoxin B (BoNT/B) that mediate the cellular entry and toxicity of BoNT/B. The BoNT/B binding domains of syt I and II are also disclosed. While syt I needs gangliosides for BoNT/B binding, syt II can bind to BoNT/B in the absence of gangliosides. Various nucleic acids and polypeptides that relate to the BoNT/B binding domain of syt I or II are disclosed. Further disclosed are methods of reducing BoNT/B toxicity, methods of identifying agents that can block the binding between BoNT/B and syt I or II, methods of identifying agents that can bind to the BoNT/B binding domain of syt I or II, methods of detecting BoNT/B or Clostridium botulinum and kits for use thereof.

Claims13

Dependent claims

A method for reducing botulinum toxin serotype B (BoNT/B) toxicity in a human or non-human animal subject comprising administering to the subject an agent that reduces binding between BoNT/B and a polypeptide that is at least 95% identical to the amino acid sequences selected from the group consisting of amino acids 32-52 of SEQ ID NO:2 (mouse synaptotagmin I botulinum toxin serotype B (BoNT/B)-binding domain), amino acids 33-53 of SEQ ID NO:5 (human synaptotagmin I botulinum toxin serotype B (BoNT/B)-binding domain), amino acids 40-60 of SEQ ID NO:7 (mouse synaptotagmin II botulinum toxin serotype B (BoNT/B)-binding domain), amino acids 40-60 of SEQ ID NO:9 (rat synaptotagmin II botulinum toxin serotype B (BoNT/B)-binding domain), and amino acids 37-57 of SEQ ID NO:10 (human synaptotagmin II botulinum toxin serotype B (BoNT/B)-binding domain).

Dependencies are inferred from the claim text.

Botulinum neurotoxin B receptors and use thereof

Life of this patent

Botulinum neurotoxin B receptors and use thereof

Life of this patent

Abstract

Claims13