Platelet aggregation test and device

An assembly for measuring platelet aggregation, comprising:

• a. an electrode subassembly comprising:
• i. an electrically non-conductive substrate with two wings at the substrate's top extending horizontally away from each other in opposite directions with a downwardly extending member;
• ii. a pair of electrode contact pads separated from each other and mounted on the wings of the substrate, each contact pad having an electrically conductive lead extending downwardly on the downwardly extending member of the substrate, a conductive wire electrically connected to each lead, the conductive wire extending below and downwardly away from the downwardly extending member, each of the two wires having a horizontal cross-section that is rounded with a cross-sectional dimension from about 0.17 to about 0.38 mm, where the portions of the two wires that extend downwardly away are substantially parallel, are spaced from one another about 0.18 to about 0.42 mm apart and are from about 1.9 to about 4.5 mm long;
• b. a cuvette subassembly comprising:
• i. a lower reservoir to receive a sample containing platelets, the lower reservoir having a substantially flat, closed bottom and having a substantially cylindrical wall, the lower reservoir having an internal volume of from about 225 to about 375 μL, the reservoir adapted to receive a sample containing platelets;
• ii. extending upwardly from the lower reservoir, an upper body comprising:
• a. a pair of arms and a back member with a slot formed between each arm and the back member, each slot adapted to receive a wing from the electrode subassembly;
• b. the dimensions of the cuvette subassembly being such that when the wings of the electrode subassembly are positioned in the slots, the two wires are positioned in the reservoir and above the bottom of the reservoir and adapted to be submerged in a sample placed in the reservoir;
• c. a channel from an upper part of the cuvette subassembly to the approximately the upper part of the reservoir, adapted to allow reagent to be placed into the sample, without substantially interfering mechanically with the electrode;
• c. a stir bar adapted to be placed in the bottom of the cuvette well and free to rotate under the influence of an outside source to create a flow of sample in the well and between the two wires of the electrode subassembly when the electrode subassembly is mounted in the cuvette subassembly, the distances between bottom ends of the two wires and the bottom of the lower reservoir being such that the stir bar can fit in that distance and still create such flow.