Cellular and viral inactivation
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsYossef Raviv, Julie M. Belanger, Mathias Viard, Robert Blumenthal, Julian W. Bess, Jr.
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Abstract
The invention involves inactivation of viral populations by treating the viral populations with a compound to crosslink proteins in the viral membrane, UV irradiation and further inactivation of the viruses using detergent(s). According to the invention, this method preserves the native structure of viral epitopes so that the inactivated viral preparations can be used in immunological compositions that will inhibit and/or prevent viral infection when administered to an animal.
Claims17
A method for inactivating a viral population, wherein the viral population comprises HIV, SIV, Ebola, or influenza, comprising:
- (a) forming a mixture by contacting the viral population with a compound that is:
- or a mixture thereof;
- (b) exposing the mixture to irradiation to crosslink viral proteins and generate a photocrosslinked viral preparation;
- and
- (c) removing lipids from viral membranes before or after steps (a) and (b) by extracting the photocrosslinked viral preparation with a detergent, to thereby inactivate the viral population.
A method for inactivating a population of HIV, SIV, Ebola, or influenza viruses comprising:
- (a) contacting the population with a compound of any of the following structures, or a mixture thereof:
- to generate a mixture of the compound(s) and the viral particles;
- (b) exposing the mixture to ultraviolet irradiation to crosslink viral membrane proteins to generate a photocrosslinked viral preparation;
- and
- (c) removing lipids from viral membranes by extracting the photocrosslinked viral preparation with an aqueous solution of an oxyethylated alkylphenol detergent;
- to thereby inactivate a population of enveloped viruses.
Dependencies are inferred from the claim text.