Methods and compositions comprising heat shock proteins
AssigneeVentria Bioscience
InventorMichael E. Barnett
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Abstract
Compositions, and uses thereof, which are beneficial for eukaryotic cells in culture, and methods for their use in promoting cell growth, viability and recombinant protein expression. The methods disclosed in the present application are useful, for example, for improving cell viability and in accelerating the rate of cell growth of cells grown in culture. In one aspect, the supplements of the invention are useful for improving or enhancing the yield of the recombinant proteins from the cell cultures.
Claims25
A cell culture medium supplement composition comprising:
- a first protein and a second protein, wherein the composition is produced in a plant, wherein said first protein is a protein cell culture component and said second protein is a heat shock protein;
- and wherein said protein cell culture component comprises at least 0.01% wt/wt of said heat shock protein;
- and wherein said protein cell culture component has less than 1 EU of endotoxin/mg of said protein cell culture component.
A supplement for use in serum free culture, comprising monomeric albumin to which is bound about 1% to about 2% wt/wt of long chain fatty acids and about 0.01% to about 0.1% wt/wt of heat shock proteins.
Dependencies are inferred from the claim text.