Non-static suspension culture of cell aggregates
AssigneesRegents of the University of Minnesota, Katholieke Universiteit Leuven
InventorsKartik Subramanian, Wei-Shou Hu, Catherine M. Verfaillie, Yonsil Park
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Abstract
The invention is directed to compositions of cell aggregates and methods for making and using the cell aggregates where the aggregates comprise cells that are not embryonic stem cells but can differentiate into cell types of at least two of ectodermal, endodermal, and mesodermal embryonic germ layers, e.g., stem cells.
Claims8
A cell culture composition comprising aggregates of stem cells in non-static cell culture wherein the cell density ranges from about 5×104 cells/ml to about 108 cells/ml, wherein the number of cells per aggregate is about 50,000 cells and the average diameter of each aggregate is about 1 mm.
Dependencies are inferred from the claim text.