Biotechnological production of chondroitin
AssigneeGnosis S.p.A.
InventorsAntonio Trilli, Immacolata Busiello, Simona Daly, Francesca Bagatin
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Abstract
Chondroitin is produced by culturing a recombinant microorganism which is obtained by inactivation of a gene encoding an enzyme responsible for addition of fructose residues to the linear chondroitin polysaccharide in a microorganism producing a fructosylated derivative of chondroitin.
Claims19
A recombinant microorganism producing chondroitin, defined as a linear glycosaminoglycan constituted by alternating residues of D-glucuronic acid (GlcUA) and N-acetyl-D-galactosamine (GalNAc) bound by β1-3 (GlcUA→GalNAc) and β1-4 (GalNAc→GlcUA) bonds, characterized in that in said microorganism the kfoE gene originally present which encodes an enzyme responsible for the addition of fructose residues to the linear chondroitin backbone is inactivated by deletion or substitution entirely or in part of said gene or disruption by insertion of an additional nucleotide sequence, and
- wherein the kfoE gene encodes a protein selected from the group consisting of:
- (A) a protein comprising the amino acid sequence of SEQ ID N°2.
Dependencies are inferred from the claim text.