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US 8,609,369 B2Granted
Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast
AssigneeE. I. Du Pont de Nemours and Company
InventorsQuinn Qun Zhu, Seung-Pyo Hong
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Abstract
Promoter regions associated with the Yarrowia lipolytica peroxisomal 2,4-dienoyl-CoA reductase (SPS19) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.
Claims9
A method for the expression of a coding region of interest in a transformed yeast cell comprising:
- a) providing a transformed yeast cell having a recombinant construct, wherein the recombinant construct comprises:
- (1) a promoter region comprising a sequence selected from the group consisting of SEQ ID NOs:
- 10, 36, 37, 38 and 39;
- and
- (2) a coding region of interest which is expressible in the yeast cell;
- wherein the promoter region is operably linked to the coding region of interest;
- and
- b) growing the transformed yeast cell of step (a) under conditions whereby the recombinant construct of step (a) is expressed.
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Dependencies are inferred from the claim text.