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US 8,609,331 B2Granted
Use of ribozymes in the detection of adventitious agents
AssigneeOnoclytics Biotech Inc.
InventorMatthew C. Coffey
Life of this patent
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Abstract
The present invention provides a method of detecting adventitious agents in a composition comprising a microorganism by using ribozyme-expressing indicator cells, as well as indicator cells useful in such detection.
Claims14
A method of detecting the presence of an adventitious agent in a composition comprising a virus which virus is not the adventitious agent wherein the non-adventitious virus contains an RNA genome or utilizes an RNA transcript to replicate, comprising:
- (a) providing a population of indicator cells that expresses a ribozyme, wherein the ribozyme is capable of specifically cleaving the RNA genome or RNA transcript of the non-adventitious virus to inhibit replication or infection of the non-adventitious virus;
- (b) contacting the indicator cells with the composition under conditions that allow for cleavage of the RNA genome or RNA transcript of the non-adventitious virus by the ribozyme;
- and
- (c) determining the effect of the composition on the indicator cells, wherein any pathogenic effect indicates the presence of an adventitious agent in the composition.
1 more element
A method of validating a composition comprising a microorganism, which microorganism is not an adventitious agent comprising:
- (a) providing a population of indicator cells that expresses a ribozyme, wherein the ribozyme is capable of specifically cleaving the RNA genome or RNA transcript of the microorganism to inhibit replication or infection of the non-adventitious microorganism;
- (b) contacting the indicator cells with the composition under conditions that allow for cleavage of the RNA genome or RNA transcript of the non-adventitious microorganism by the ribozyme;
- and
- (c) determining the effect of the composition on the indicator cells, wherein the absence of any pathogenic effect validates the composition as having no detectable adventitious agent.
1 more element
Dependencies are inferred from the claim text.