Methods and compositions for producing vitamin K dependent proteins
AssigneeThe University of North Carolina at Chapel Hill
InventorsDarrel W. Stafford, Tao Li
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Abstract
The present invention relates to methods and compositions for improving the productivity of recombinant vitamin K dependent protein expression in host cells.
Claims24
An isolated host cell comprising a recombinant nucleic acid coding for a functional vitamin K epoxide reductase (VKOR), wherein the recombinant nucleic acid coding for the VKOR comprises a nucleotide sequence selected from the group consisting of:
- 1) a nucleotide sequence that encodes an amino acid sequence having at least 80% identity with the amino acid sequence of SEQ ID NO:10 and that encodes a VKOR;
- 2) a nucleotide sequence that that encodes a human VKOR;
- 3) a nucleotide sequence that encodes a mouse VKOR;
- 4) a nucleotide sequence that encodes a rat VKOR;
- 5) a nucleotide sequence that encodes a zebrafish VKOR;
- 6) a nucleotide sequence that encodes a Xenopus VKOR;
- and
- 7) a nucleotide sequence that encodes an Anopheles VKOR,
- and a recombinant nucleic acid coding for a vitamin K dependent protein, wherein both the recombinant VKOR and the recombinant Vitamin K dependent protein are expressed in said host cell.
A cell culture system comprising isolated cells that contain a recombinant nucleic acid coding for a functional vitamin K epoxide reductase (VKOR), wherein the recombinant nucleic acid coding for the VKOR comprises a nucleotide sequence selected from the group consisting of:
- 1) a nucleotide sequence that encodes an amino acid sequence having at least 80% identity with the amino acid sequence of SEQ ID NO:10 and that encodes a VKOR;
- 2) a nucleotide sequence that that encodes a human VKOR;
- 3) a nucleotide sequence that encodes a mouse VKOR;
- 4) a nucleotide sequence that encodes a rat VKOR;
- 5) a nucleotide sequence that encodes a zebrafish VKOR;
- 6) a nucleotide sequence that encodes a Xenopus VKOR;
- and
- 7) a nucleotide sequence that encodes an Anopheles VKOR, and a recombinant nucleic acid coding for a vitamin K dependent protein, wherein both the recombinant VKOR and the recombinant Vitamin K dependent protein are expressed in said cells.
Dependencies are inferred from the claim text.