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US 8,603,811 B2Granted
Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
AssigneeViaCyte, Inc.
InventorsKevin A. D'Amour, Anne Bang, Emmanuel E. Baetge
Life of this patent
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Abstract
Disclosed herein are cell cultures and enriched cell populations of endocrine precursor cells, immature pancreatic hormone-expressing cells and mature pancreatic hormone-expressing cells. Also disclosed herein are methods of producing such cell cultures and cell populations.
Claims18
A first and second cell population comprising:
- a) a first in vitro cell population comprising human pluripotent cells;
- and
- b) a second in vitro cell population comprising progeny of a portion of the first cell population, wherein the progeny comprise at least 5% endocrine precursor cells that express a marker selected from the group consisting of neurogenin 3 (NEUROG3), paired box 4 (PAX4) and NKX2 transcription factor related locus 2 (NKX2.2).
A first and second cell population comprising
- a) a first in vitro cell population comprising human pluripotent cells;
- and
- b) a second in vitro cell population comprising progeny of a portion of the first cell population, wherein the progeny are obtained by:
- (1) providing said first in vitro cell population with a TGFβ superfamily growth factor;
- (2) withdrawing the TGFβ superfamily growth factor from said cell population;
- (3) providing said cell population with a retinoid;
- and
- (4) incubating said cell population in a culture medium for a sufficient time to permit formation of human endocrine precursor cells expressing a marker selected from the group consisting of neurogenin 3 (NEUROG3), paired box 4 (PAX4) and NKX2 transcription factor related locus 2 (NKX2.2).
5 more elements
Dependencies are inferred from the claim text.