Nucleic acid sample preparation
AssigneeBiological Dynamics, Inc.
InventorsRajaram Krishnan, David Charlot, Eugene Tu, James McCanna, Lucas Kumosa, Paul Swanson, Robert Turner, Kai Yang, Irina Dobrovolskaya, David Liu
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Abstract
The present invention includes methods, devices and systems for isolating a nucleic acid from a fluid comprising cells. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity nucleic acid isolated from complex fluids such as blood or environmental samples.
Claims30
A method for isolating a nucleic acid from a fluid comprising cells, the method comprising:
- a. applying the fluid to a device, the device comprising an array of electrodes capable of establishing an AC electrokinetic field region;
- b. concentrating a plurality of cells in a first AC electrokinetic field region, wherein the first AC eletrokinetic field region is a first dielectrophoretic high field region and the conductivity of the fluid is less than 500 mS/m;
- c. lysing the cells on the array;
- and
- d. isolating nucleic acid in a second AC electrokinetic field region, wherein the second AC electrokinetic field is a second dielectrophoretic high field region.
A method for isolating a nucleic acid from a fluid comprising cells, the method comprising:
- a. applying the fluid to a device, the device comprising an array of electrodes capable of establishing an AC electrokinetic field region;
- b. concentrating a plurality of cells in a first AC electrokinetic field region, wherein the first AC electrokinetic field region is a first dielectrophoretic low field region and the conductivity of the fluid is greater than 300 mS/m;
- c. isolating nucleic acid in a second AC electrokinetic field region, wherein the second AC electrokinetic field is a second eletrophoretic high field region;
- and
- d. flushing cells away from the array.
The method of 16, further comprising degrading residual proteins on the array.
Dependencies are inferred from the claim text.