Method of cell cultures and device for implementing it

A cell culture device comprising:

• a culture vessel provided with a cover, wherein there is situated at least one first zone and at least one second zone, wherein said first zone is for transferring the culture medium essentially containing no cells and said second zone is for culturing cells, means of circulating the culture medium, allowing circulation of the culture medium through the second zone, said second zone comprising a bottom wall and a top wall, wherein each wall is provided with orifices allowing a transfer of culture medium essentially free from cells, and further comprising at least one third and at least one fourth zone, wherein both are essentially free from cells, wherein said third zone is in medium communication with the first and second zones and wherein said fourth zone is in medium communication with the second zone and with the first zone via the means for circulating the culture medium, and in that the means for circulating is adapted to circulate the culture medium from bottom to top in said second zone, wherein said third zone includes an overflow so that the culture medium overflows from the first zone to the third zone.