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US 8,597,916 B2Granted
Multimeric oxidoreductases
AssigneeDanisco US Inc.
InventorsRobert M. Caldwell, M. Harunur Rashid, Fernando Valle
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Abstract
The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.
Claims10
A method for producing a desired product comprising,
- contacting an altered bacterial host cell with a polyol substrate under suitable culture conditions, wherein the altered bacterial host cell is obtained by the transformation of a bacterial host cell with a polynucleotide coding for a multimeric oxidoreductase complex selected from:
- SEQ ID NO:
- 19 and SEQ ID NO:
- 38
- and wherein the polynucleotide encoding the multimeric oxidoreductase complex is expressed in the altered bacterial host cell and
- producing a desired product from an enzymatic conversion of the polyol substrate by the multimeric oxidoreductase complex to said desired product.
3 more elements
Dependencies are inferred from the claim text.