Glycosylation of molecules
AssigneesOxyrane UK Limited, Vib Vzw, Universiteit Gent
InventorsNico Luc Marc Callewaert, Wouter Vervecken, Karen Jacqueline Marcel De Pourcq, Steven Christian Jozef Geysens, Mouna Guerfal
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Abstract
Described herein are methods and genetically engineered cells useful for producing an altered N-glycosylation form of a target molecule. Also described are methods and molecules with altered N-glycosylation useful for treating a variety of disorders such as metabolic disorders.
Claims38
A method of producing an altered N-glycosylation form of a target protein, the method comprising:
- providing a Yarrowia lipolytica or an Arxula adeninivorans cell genetically engineered to express a protein capable of removing glucose residues from GlcMan5GlcNAc2 or Glc2Man5GlcNac2 and to comprise a deficiency in an Asparagine Linked Glycosylation 3 (ALG3) activity;
- and
- introducing into the cell a nucleic acid encoding a target protein, wherein the cell produces the target protein in an altered N-glycosylation form relative to the N-glycosylation form of the target protein produced in a corresponding non-genetically engineered cell.
An isolated Yarrowia lipolytica or Arxula adeninivorans cell genetically engineered to express a protein capable of removing glucose residues from GlcMan5GlcNAc2 or Glc2Man5GlcNAc2 and to comprise a deficiency in an ALG3 activity.
A method of producing an altered N-glycosylation form of a target protein, the method comprising contacting a target protein with a cell lysate prepared from a Yarrowia lipolytica or an Arxula adeninivorans cell genetically engineered to express a protein capable of removing glucose residues from GlcMan5GlcNAc2 or Glc2Man5GlcNAc2 and to comprise a deficiency in an ALG3 activity, wherein the contacting of the target protein with the cell lysate results in an altered N-glycosylation form of the target protein relative to the N-glycosylation form of the target protein produced from the cell lysate of a corresponding non-genetically engineered cell.
A substantially pure culture of Yarrowia lipolytica or Arxula adeninivorans cells, wherein the culture comprises Yarrowia lipolytica or Arxula adeninivorans cells which are genetically engineered to express a protein capable of removing glucose residues from GlcMan5GlcNAc2 or Glc2Man5GlcNAc2 and to comprise a deficiency in an ALG3 activity.
Dependencies are inferred from the claim text.