US 8,592,642 B2Granted

Evaluation/screening method for diseases associated with D-amino acid utilizing DAO1-/-mouse

AssigneesKyushu University, National University Corporation, Shiseido Company, Ltd.

InventorsKenji Hamase, Kiyoshi Zaitsu, Masashi Mita, Yutaka Ashida, Yousuke Toujo

Life of this patent

Estimating the term…

Loading patent…

Abstract

Disclosed is an evaluation method which can rapidly discriminate a Dao−/− homozygote from a large number of animals produced in a mating experiment between a DAO enzyme deficient mouse and other disease model mice, to rapidly perform a quantitative measurement of the D-amino acids contained in a large number of samples. The invention provides a method for evaluating the effect of a test condition on a mouse tissue, or cultured tissue cells derived from the tissue. The method comprises the steps of: providing a Dao1−/− mouse or the like; exposing the tissue from the Dao1−/− mouse or the like, to the test condition; and analyzing the effect of exposing the tissue from the Dao1−/− mouse or the like, to the test condition.

Claims12

Dependent claims

A method for evaluating an effect of a transplanted tumor on a mouse tissue, or cultured cells derived from the tissue, said method comprising the steps of:

(1) providing a Dao 1+/+ mouse and a Dao 1−/− mouse,
(2) exposing a tissue of the Dao 1+/+ and Dao 1−/− mice, or cultured tissue cells derived from the tissue to the transplanted tumor, and
(3) comparing changes detected in the tissue or cultured tissue cells derived from the tissue of the Dao 1+/+ and Dao 1−/− mice exposed to the transplanted tumor with tissue or cultured tissue cells derived from the tissue of the Dao 1+/+ and Dao 1−/− mice that were not exposed to the transplanted tumor, wherein the mice of step (1) are provided by the following steps:
(a) identifying a Dao 1+/+, +/− and/or −/− mouse individually,
(b) extracting chromosomal DNA from the mouse (a),
(c) obtaining an amplified DNA fragment by amplifying the region covering the seventh exon of the Dao 1 gene from the extracted chromosomal DNA,
(d) digesting the amplified DNA fragment with a HpaII restriction enzyme and examining whether the amplified DNA fragment is cleaved or not,
(e) determining a genotype of Dao 1+/+, +/− and/or −/−;
and
(f) discriminating the Dao 1+/+ mouse from the Dao−/− mouse.

7 more elements

Dependencies are inferred from the claim text.