Method for making antibody Fc-heterodimeric molecules using electrostatic steering effects
AssigneeAmgen Inc.
InventorsGunasekaran Kannan, Michael Wittekind, Wei Yan, Martin Pentony
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Abstract
The invention relates to methods of making Fc-heterodimeric proteins or polypeptides. The invention also relates to the Fc-heterodimeric proteins or polypeptides themselves, including the individual polypeptide components that comprise the heterodimer. Nucleic acids encoding such polypeptides, expression vectors, and host cells. Moreover, the invention relates to pharmaceutical compositions comprising one of more Fc-heterodimeric proteins or polypeptides.
Claims18
An isolated heterodimeric protein comprising a first human CH3-containing polypeptide and a second human IgG CH3-containing polypeptide wherein said first human CH3-containing polypeptide comprises a replacement of the amino acid at position 392 with a negative-charged amino acid and said second human IgG CH3-containing polypeptide comprises a replacement of Asp399, Glu356, Asp356, or Glu357 with a positive-charged amino acid.
Dependencies are inferred from the claim text.