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AssigneeSolazyme, Inc.
InventorsScott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky
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Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
An oil composition produced by cultivating an oleaginous microbe and recovering the oil composition from the microbe, the oil composition comprising triglycerides and sterols produced by the microbe, wherein the fatty acid profile of the triglyceride oil composition comprises at least 60% C18:1, and less than 7% C18:2, wherein the triglyceride oil composition is produced by a recombinant oleaginous microbe comprising an exogenous gene, and optionally wherein an endogenous desaturase of the oleaginous microbe has been inactivated or mutated to have less enzymatic activity.
An oil composition produced by cultivating an oleaginous microalgae and recovering the oil composition from the microalgae, the oil composition comprising triglycerides and sterols produced by the microalgae, wherein the fatty acid profile of the triglyceride oil composition comprises at least 60% C18:1, and less than 7% C18:2, wherein the triglyceride oil composition is produced by a recombinant oleaginous microalgae comprising an exogenous gene, and optionally wherein an endogenous desaturase of the oleaginous microalgae has been inactivated or mutated to have less enzymatic activity.
An oil composition, produced by cultivating an oleaginous microbe and recovering the oil composition from the microbe, the oil composition comprising triglycerides and sterols produced by the microbe, wherein the fatty acid profile of the triglyceride oil composition comprises at least 50% saturated fatty acids, wherein the triglyceride oil composition is produced by a recombinant oleaginous microbe comprising an exogenous gene, and optionally wherein an endogenous desaturase of the oleaginous microbe has been inactivated or mutated to have less enzymatic activity.
Dependencies are inferred from the claim text.