Methods of producing GABA
AssigneePlant Sensory Systems, LLC
InventorsFrank J. Turano, Kathleen A. Turano
Life of this patent
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Abstract
The present invention describes an alternative approach to increase GABA production in prokaryotes or eukaryotes, namely by the insertion of the putrescine catabolic pathway in organisms where the pathway does not exist or has not clearly been identified. The invention describes methods for the use of polynucleotides that encode functional putrescine aminotransferase (PAT) and gamma-aminobutyricaldehyde dehydrogenase (GABAlde DeHase) polypeptides in plants to increase GABA production. The preferred embodiment of the invention is in plants but other organisms may be used. Changes in GABA availability will improve growth and increase tolerance to biotic and abiotic stress.
Claims39
A plant cell comprising either:
- (a) two expression units, wherein
- (i) a first expression unit comprises a first promoter operably linked to a first polynucleotide which encodes putrescine aminotransferase comprising the amino acid sequence set forth in SEQ ID NO:52;
- and
- ii) a second expression unit comprises a second promoter operably linked to a second polynucleotide which encodes gamma-aminobutyricaldehyde dehydrogenase comprising the amino acid sequence set forth in SEQ ID NO:60;
- or
- (b) a single expression unit which comprises a third promoter operably linked to a polynucleotide which encodes putrescine aminotransferase comprising the amino acid sequence set forth in SEQ ID NO:52.
A method for producing GABA, comprising incorporating into a plant cell either:
- (a) two expression units, wherein
- (i) a first expression unit comprises a first promoter operably linked to a first polynucleotide which encodes putrescine aminotransferase comprising the amino acid sequence set forth in SEQ ID NO:52;
- and
- (ii) a second expression unit comprises a second promoter operably linked to a second polynucleotide which encodes gamma-aminobutyricaldehyde dehydrogenase comprising the amino acid sequence set forth in SEQ ID NO:60;
- or
- (b) a single expression unit which comprises a promoter operably linked to a polynucleotide which encodes putrescine aminotransferase aminotransferase comprising the amino acid sequence set forth in SEQ ID NO:52,
- whereby the plant cell produces GABA.
Dependencies are inferred from the claim text.