Process for the identification and preparation of a (R)-specific omega-transaminase

A process for the preparation and screening of a (R)-selective ω-transaminase, comprising the following steps:

• a) providing at least one (R)-selective ω-transaminase sequence and at least one biomolecule bank,
• b) searching the biomolecule bank with the (R)-selective ω-transaminase sequence to identify a group of first target biomolecule sequences, wherein the first target biomolecule sequences have a degree of sequence identity of at least 20% to the query biomolecule sequence, calculated on amino acid level,
• c) selecting in the group of first target biomolecule sequences a group of second target biomolecule sequences, which do not comprise, on amino acid level, at least one of the following amino acid sequence motives c1) to c3) with
• c1) at position 95 to 97 an amino acid sequence Tyr Xa1 Xa2, with Xa1 being an amino acid Ile, Val, Leu, Met, Phe, and Xa2 being an amino acid Arg or Lys or
• c2) at position 97 to 99 an amino acid sequence Tyr Xaa Gln, with Xaa being an amino acid and in the region from position 105 to 111 an amino acid sequence Arg Xaa Xa3, Xa3 being an amino acid, preferably being His or
• c3) at position 38 Thr, at position 97 Lys and at position 107 to 109 an amino acid sequence Arg Xa4 Xa5, Xa4 being an amino acid, preferably being Gly and Xa5 being an amino acid, preferably being Tyr
• and which comprise
• c4) at position 95 an amino acid other than Tyr, Arg, Lys, or at position 95 Tyr, but at position 97 no Arg or Lys and
• c5) at position 40 no Lys or Arg and
• c6) in the region from position 161 to 165, a Lys to identify the group of second target biomolecule sequences;
• and
• d) providing an isolated biomolecule having or encoding one of the second target biomolecule sequences identified in step c);
• e) testing whether the isolated protein biomolecule has an (R)-selective ω-transaminase activity.