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AssigneeMonsanto Invest B.V.
InventorsJohannes Arnoldus Laurentius van Kan, Arjen ten Have, Willem Hendrik Lindhout, Hendrikus Johannes Finkers, Remco van Berloo, Adriaan Willem van Heusden
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The present invention relates to a method for detecting a quantitative trait locus (QTL) associated with resistance to Botrytis cinerea in tomato, including the steps of crossing a Botrytis-resistant donor tomato plant with a non-resistant, or Botrytis-susceptible, recipient tomato plant, contacting one or more offspring plants with an infective amount of Botrytis, quantitatively determining the disease incidence and/or the rate of lesion growth in the one or more offspring plants, establishing a genetic linkage map that links the observed disease incidence and/or rate of lesion growth to the presence of chromosomal markers of the donor tomato plant in the one or more offspring plants, and assigning to a QTL the contiguous markers on the map that are linked to a reduced disease incidence and/or a reduced lesion growth rate.
A Botrytis-resistant tomato plant, comprising at least one quantitative trait locus (QTL) associated with Botrytis-resistance of Lycopersicon parviflorum G1.1601, said QTL being selected from the group consisting of QTL-3p , QTL-4p , and QTL-9p , wherein said QTL of Lycopersicon parviflorum G1.1601 is not in the natural genetic background of the Botrytis-resistant tomato plant,
A method of producing a Botrytis-resistant tomato plant comprising the step of transferring a nucleic acid comprising at least one QTL associated with Botrytis resistance of Lycopersicon parviflorum G1.1601 from a Botrytis-resistant donor tomato plant to a Botrytis-susceptible recipient tomato plant, wherein said transfer of said nucleic acid is performed by transformation, by crossing, by protoplast fusion, by a doubled-haploid technique or by embryo rescue,
A Botrytis-resistant tomato plant, or part thereof, comprising within its genome at least one QTL wherein said QTL is selected from the group consisting of the QTLs on chromosomes 3, 4, and 9 in Lycopersicon parviflorum G1.1601 associated with Botrytis-resistance, wherein said QTL is not in its natural genetic background, and
Dependencies are inferred from the claim text.