Maize event DP-004114-3 and methods for detection thereof
AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsScott Diehn, Albert L. Lu, Timothy M. Nowatzki, Douglas S. Nubel, M. Alejandra Pascual, James C. Register, III, Christopher J. Scelonge, Joshua K. Young, Cathy Xiaoyan Zhong, Erin L. Crowgey
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Abstract
The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
Claims28
A DNA construct comprising:
- a first, second, third and fourth expression cassette, wherein said first expression cassette in operable linkage comprises:
- (a) a maize ubiquitin promoter;
- (b) a 5′ untranslated exon of a maize ubiquitin gene;
- (c) a maize ubiquitin first intron;
- (d) a Cry1F encoding DNA molecule;
- and
- (e) a poly(A) addition signal from ORF 25 terminator;
- said second expression cassette in operable linkage comprises:
- (1) a maize ubiquitin promoter;
- (2) a 5′ untranslated exon of a maize ubiquitin gene;
- (3) a maize ubiquitin first intron;
- (4) a Cry34Ab1 encoding DNA molecule;
- and
- (5) a PinII transcriptional terminator;
- said third expression cassette in operable linkage comprises;
- (i) a wheat peroxidase promoter;
- (ii) a Cry35Ab1 encoding DNA molecule;
- and
- (iii) a PinII transcriptional terminator;
- and
- said fourth expression cassette in operable linkage comprises;
- (a) a CaMV 35S promoter;
- (b) a pat encoding DNA molecule;
- and
- (c) a 3′ transcriptional terminator from CaMV 35S;
- wherein the four cassettes are flanked by SEQ ID NO:
- 27 at the 5′ end and SEQ ID NO:
- 28 at the 3′ end.
A plant comprising the sequence set forth in SEQ ID NO:
- 6.
A corn plant comprising the genotype of the corn event DP-004114-3, wherein a representative sample of seed of said corn event has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506.
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 6;
- SEQ ID NO:
- 27;
- SEQ ID NO:
- 28, and full length complements thereof.
An amplicon comprising the nucleic acid sequence selected from the group consisting of SEQ ID NO:
- 27, SEQ ID NO:
- 28 and full length complements thereof.
A biological sample derived from corn event DP-004114-3 plant, tissue, or seed, wherein said sample comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 27 and SEQ ID NO:
- 28, or the complement thereof, wherein said nucleotide sequence is detectable in said sample using a nucleic acid amplification or nucleic acid hybridization method, wherein a representative sample of said corn event DP-004114-3 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506.
An extract derived from corn event DP-004114-3 plant, tissue, or seed and comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 27 and SEQ ID NO:
- 28 or the complement thereof, wherein a representative sample of said corn event DP-004114-3 seed has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506.
A method for producing a corn plant resistant to at least corn rootworm, said method comprising:
- (a) sexually crossing a first parent corn plant with a second parent corn plant, wherein said first or second parent corn plant is a corn event DP-004114-3, deposited under Accession No. PTA-11506 with American Type Culture Collection (ATCC), plant, thereby producing a plurality of first generation progeny plants;
- (b) selecting a first generation progeny plant that is resistant to at least corn rootworm infestation;
- (c) backcrossing the first generation progeny plant of step (b) with the parent plant that lacks corn event DP-004114-3 DNA, thereby producing a plurality of backcross progeny plants;
- and
- (d) selecting from the backcross progeny plants, a plant that is resistant to at least corn rootworm infestation;
- wherein the selected backcross progeny plant of step (d) comprises SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.