Bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof

A transgenic plant having stably incorporated in its genome a transgene, wherein said transgene comprises a polynucleotide operably linked to a promoter functional in a plant cell, and wherein the polynucleotide comprises at least one of

• a. a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 17;
• b. a polynucleotide comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:
• 18;
• or
• c. a polynucleotide that encodes a polypeptide having insecticidal activity and at least 99% sequence identity to the amino acid sequence of SEQ ID NO:
• 18.

A method for producing a plant with increased insect resistance, the method comprising:

• a. introducing into plant cells a construct comprising a polynucleotide encoding an insecticidal polypeptide operably linked to a promoter functional in plant cells to yield transformed plant cells, and wherein the polynucleotide encoding the insecticidal polypeptide is selected from the group consisting of:
• i. a polynucleotide that encodes the amino acid sequence of SEQ ID NO:
• 18;
• ii. a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
• 17;
• and
• (iii) a polynucleotide that encodes a polypeptide having insecticidal activity and at least 99% sequence identity to the amino acid sequence of SEQ ID NO:
• 18;
• and
• b. regenerating a transgenic plant from said transformed plant cells, wherein said insecticidal polypeptide is expressed at levels sufficient to increase insect resistance in said transgenic plant as compared to a control plant.

An isolated polynucleotide comprising a member selected from the group consisting of:

• a. a nucleotide sequence that encodes the amino acid sequence set forth in SEQ ID NO:
• 18;
• and
• b. a nucleotide sequence that encodes a polypeptide having insecticidal activity and at least 99% sequence identity to the amino acid sequence set forth in SEQ ID NO:
• 18.