Production of functionalized linear DNA cassette and quantum dot/nanoparticle mediated delivery in plants
AssigneeDow AgroSciences LLC
InventorsKerrm Y. Yau, Jayakumar P. Samuel, Frank Burroughs, Narasimha C. Samboju, Steven R. Webb
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Abstract
Methods for introducing a functionalized linear nucleic acid cassette molecule of interest into a plant cell comprising a cell wall include use of nanoparticles. In some embodiments, the cell comprising a cell wall is a cultured plant cell. Methods include genetically or otherwise modifying plant cells and for treating or preventing disease in any plant, especially crop plants. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with functionalized linear nucleic acid cassette molecules.
Claims20
A method of introducing a functionalized linear nucleic acid cassette molecule of interest into a plant cell having a cell wall, the method comprising:
- providing the plant cell having a cell wall;
- coating a quantum dot nanoparticle with a biotinylated linear nucleic acid cassette molecule of interest, wherein the quantum dot nanoparticle comprises streptavidin that interacts with the biotinylated linear nucleic acid cassette molecule of interest;
- placing the plant cell having a cell wall and the coated quantum dot nanoparticle in contact with each other;
- allowing uptake of the quantum dot nanoparticle and the biotinylated linear nucleic acid cassette molecule of interest into the plant cell comprising a cell wall;
- and selecting cells that have stably integrated the linear nucleic acid cassette molecule of interest.
A method of introducing a functionalized linear nucleic acid cassette molecule of interest into plant material, the method comprising:
- providing plant material, wherein the plant material is selected from the group consisting of plant cells, plant tissues, and plants;
- providing a quantum dot nanoparticle, wherein the quantum dot nanoparticle comprises streptavidin that interacts with a biotinylated linear nucleic acid cassette molecule of interest;
- coating the quantum dot nanoparticle with the biotinylated linear nucleic acid cassette molecule of interest;
- placing the cell having a cell wall and the coated quantum dot nanoparticle in contact with each other;
- allowing uptake of the quantum dot nanoparticle and the biotinylated linear nucleic acid cassette molecule of interest into the plant material;
- and selecting cells that have stably integrated the linear nucleic acid cassette molecule of interest.
A method for introgressing a trait into a plant, the method comprising:
- providing a plant cell;
- providing a quantum dot nanoparticle, wherein the quantum dot nanoparticle comprises streptavidin that interacts with a biotinylated linear nucleic acid cassette molecule for expressing the trait in the plant;
- coating the quantum dot nanoparticle with the biotinylated linear nucleic acid cassette molecule for expressing the trait in the plant;
- placing the plant cell and the coated quantum dot nanoparticle in contact with each other;
- allowing uptake of the coated quantum dot nanoparticle into the plant cell;
- regenerating a whole plant from the transformed plant cell;
- and propagating the plant;
- wherein the propagated plant has stably integrated said trait.
Dependencies are inferred from the claim text.