High-purity fractionation of anthocyanins from fruits and vegetables
AssigneeThe Ohio State University Research Foundation
InventorsMaria Monica Giusti, Jian He
Life of this patent
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Abstract
Disclosed is a method for separating anthocyanins depleted in phenolic mixture content from fruits or vegetables feedstock containing anthocyanins and phenolic mixtures. The first step is to contact the feedstock with a cation-exchange resin at low pH for a time period effective for the resin to selectively bind with the anthocyanins. Next, the non-bound phenolic mixture is separated from the resin for recovery. The bound resin is subjected to solvent wash to release the anthocyanins for recovery.
Claims15
A method for separating anthocyanins from plant tissue feedstock containing anthocyanins and phenolic mixtures, which comprises the sequential steps of:
- (a) contacting said feedstock with a mixed mode cation-exchange resin for a time period effective for said resin to selectively bind with said anthocyanins;
- (b) separating non-bound phenolic mixture from said resin;
- (c) subjecting bound resin to alkaline solvent wash to release said anthocyanins in an eluate;
- (d) mixing the eluate with an acid such that a resulting mixture has pH less than about pH 2;
- and
- (e) recovering, from the resulting mixture, anthocyanins having a purity of at least 85%.
A method for purifying anthocyanins comprising sequentially:
- providing an anthocyanin-containing supernatant;
- adsorbing anthocyanins from the supernatant onto a resin under acid conditions;
- washing the resin with a first solvent;
- eluting the anthocyanins from the resin with a second solvent to produce an anthocyanin-rich eluate, wherein the second solvent is alkaline, wherein the second solvent is a food-grade solvent having pH between 7 and 8.5;
- mixing the eluate with an acid such that the resulting mixture has a pH of less than about 2;
- and
- recovering, from the resulting mixture, anthocyanins having a purity of at least 85%.
Dependencies are inferred from the claim text.