Formicidae (ant) control using double-stranded RNA constructs
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsRobert K. Vander Meer, Man Yeon Choi
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Abstract
Disclosed is a dsRNA construct that relates to a method to control Formicidae (ants) via double-stranded RNA interference of the PBAN/Pyrokinin gene.
Claims17
An isolated double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a pheromone-biosynthesis-activating neuropeptide/pyrokynin peptide in a cell consisting essentially of sense strand SEQ. ID. NO. 2 with the sense strand being complementary to an antisense strand.
An isolated double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a pheromone-biosynthesis-activating neuropeptide/pyrokynin peptide in a cell consisting essentially of sense strand SEQ. ID. NO. 3 with the sense strand being complementary to an antisense strand.
A method for controlling Solenopsis invicta, the method comprising:
- constructing a double-stranded ribonucleic acid construct that is complementary to a gene that encodes a PBAN/Pyrokinin gene sequence consisting essentially of SEQ. ID. NO. 2 and 3, dissolving the double-stranded ribonucleic acid to form a solution, and contacting an effective amount of said solution to Solenopsis invicta, wherein said solution is ingested by Solenopsis invicta and RNA interference is induced, resulting in disruption of critical developmental and/or reproductive functions of Solenopsis invicta.
Dependencies are inferred from the claim text.