Antibody against oxidized LDL/β2GPI complex and use of the same
AssigneesNational University Corporation Okayama University, Medical & Biological Laboratories Co., Ltd.
InventorsEiji Matsuura, Kazuo Kojima
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Abstract
From antibodies that can be used to immunostain atherosclerotic tissue sections, the present inventors selected antibodies applicable to in vivo imaging, and analyzed their specificities. The result showed that fluorescently labeled anti-oxidized LDL/β2GPI complex antibodies that are specific to a particular epitope were effective for imaging.
Claims11
An isolated antibody that binds to a complex of oxidized LDL and β2-glycoprotein I (oxidized LDL/β2GPI complex) and comprises a pair of the heavy chain described in (a) or (b) below and the light chain described in (c) or (d) below:
- (a) an antibody comprising a heavy chain that comprises CDR1 having the amino acid sequence of SEQ ID NO:
- 2, CDR2 having the amino acid sequence of SEQ ID NO:
- 3, and CDR3 having the amino acid sequence of SEQ ID NO:
- 4;
- (b) an antibody comprising a heavy chain that comprises a heavy chain variable region having the amino acid sequence of SEQ ID NO:
- 1;
- (c) an antibody comprising a light chain that comprises CDR1 having the amino acid sequence of SEQ ID NO:
- 7, CDR2 having the amino acid sequence of SEQ ID NO:
- 8, and CDR3 having the amino acid sequence of SEQ ID NO:
- 9;
- (d) an antibody comprising a light chain that comprises a light chain variable region having the amino acid sequence of SEQ ID NO:
- 6.
An isolated antibody that binds to the same epitope as an antibody that binds to a complex of oxidized LDL and β2-glycoprotein I (oxidized LDL/β2GPI complex), and which comprises a pair of the heavy chain described in (a) or (b) below and the light chain described in (c) or (d) below:
- (a) an antibody comprising a heavy chain that comprises CDR1 having the amino acid sequence of SEQ ID NO:
- 2, CDR2 having the amino acid sequence of SEQ ID NO:
- 3, and CDR3 having the amino acid sequence of SEQ ID NO:
- 4;
- (b) an antibody comprising a heavy chain that comprises a heavy chain variable region having the amino acid sequence of SEQ ID NO:
- 1;
- (c) an antibody comprising a light chain that comprises CDR1 having the amino acid sequence of SEQ ID NO:
- 7, CDR2 having the amino acid sequence of SEQ ID NO:
- 8, and CDR3 having the amino acid sequence of SEQ ID NO:
- 9;
- (d) an antibody comprising a light chain that comprises a light chain variable region having the amino acid sequence of SEQ ID NO:
- 6.
An imaging agent for visualizing an arteriosclerosis site, which comprises an isolated antibody that binds to a complex of oxidized LDL and β2-glycoprotein I (oxidized LDL/β2GPI complex), and which comprises a pair of the heavy chain described in (a) or (b) below and the light chain described in (c) or (d) below:
- (a) an antibody comprising a heavy chain that comprises CDR1 having the amino acid sequence of SEQ ID NO:
- 2, CDR2 having the amino acid sequence of SEQ ID NO:
- 3, and CDR3 having the amino acid sequence of SEQ ID NO:
- 4;
- (b) an antibody comprising a heavy chain that comprises a heavy chain variable region having the amino acid sequence of SEQ ID NO:
- 1;
- (c) an antibody comprising a light chain that comprises CDR1 having the amino acid sequence of SEQ ID NO:
- 7, CDR2 having the amino acid sequence of SEQ ID NO:
- 8, and CDR3 having the amino acid sequence of SEQ ID NO:
- 9;
- (d) an antibody comprising a light chain that comprises a light chain variable region having the amino acid sequence of SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.