Sample preparation device
AssigneeAkonni Biosystems, Inc.
InventorsChristopher G. Cooney, Phillip Belgrader
Life of this patent
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Abstract
A sample preparation device is disclosed. The sample preparation device includes a housing defining a passage way between a first opening and a second opening; and a sample filter occupying a section of said passage way. The sample filter contains a monolith adsorbent that specifically binds to nucleic acids. Also disclosed are sample filters containing glass frit is coated with an capture agent that binds specifically to an analyte of interest, sample filters containing a hydrophilic matrix with impregnated chemicals that lyses cell membranes, a cartridge base and an integrated sample preparation cartridge.
Claims17
A method for purifying an analyte from a liquid sample, comprising:
- placing said liquid sample in a container;
- withdrawing at least a portion of said liquid sample into a sample preparation device comprising:
- a housing defining a passage way between a first opening and a second opening;
- and
- a filter occupying a section of said passage way, said filter comprising a material that specifically binds to said analyte,
- wherein said portion of liquid sample is drawn into said housing via the first opening and passing through said filter, wherein said filter comprises a glass frit, and wherein said analyte is a nucleic acid that binds to said glass frit while passing through said filter;
- expelling said portion of liquid sample from said sample preparation device via the first opening, wherein said portion of liquid sample passes through said filter a second time while exiting said sample preparation device;
- and
- eluting said analyte from said filter by withdrawing an eluting buffer into said sample preparation device via the first opening and expelling said eluting buffer from said sample preparation device via the first opening, wherein said eluting buffer passes through said filter while entering and exiting said sample preparation device.
A method for purifying an analyte from a sample, comprising:
- passing said sample through a sample preparation device comprising:
- a housing defining a passage way between a first opening and a second opening;
- and
- a sample filter occupying a section of said passage way,
- wherein said sample filter comprises a glass frit that specifically binds to said analyte and said sample passes through said sample filter while passing through said sample preparation device;
- and
- eluting analyte bound to said sample filter with an eluting solution,
- wherein said sample and said eluting solution enter and exit said housing through the same opening.
Dependencies are inferred from the claim text.