Method for measurement of hemoglobin and hemoglobin derivative, and measurement kit

A method for measuring a hemoglobin derivative, comprising:

• treating a sample containing a blood component including red blood cells with a nonionic surfactant, an oxidizing agent and a metal salt for denaturing so as to allow measurement of the hemoglobin and hemoglobin A1c as a hemoglobin derivative,
• performing an optical measurement of the hemoglobin in the sample,
• performing an immunoassay of the hemoglobin A1c as a hemoglobin derivative by using an antibody specifically binding to a denatured site of the hemoglobin A1c as a hemoglobin derivative denatured, and
• determining an amount of the hemoglobin derivative in the sample based on an amount of binding of the antibody to the denatured site of the hemoglobin A1c, the amount of the binding of the antibody to the denatured site of the hemoglobin A1c correlating directly to the amount of hemoglobin derivative,
• wherein the metal salt is present in an amount sufficient to accelerate the denaturation of the hemoglobin and includes at least one metal ion selected from the group consisting of sodium (Na), potassium (K), magnesium (Mg) and calcium (Ca).

An analysis device for use in measuring hemoglobin in a sample containing a blood component including red blood cells, comprising:

• a sample addition site for adding the sample;
• a denaturation section connected to the sample addition site, for denaturing the hemoglobin in the added sample with a reagent composition containing a nonionic surfactant, an oxidizing agent and a metal salt, wherein the denaturation section comprises the reagent composition, and
• a detection section connected to the denaturation section, for detecting the denatured hemoglobin,
• wherein the metal salt included in the reagent composition is present in an amount sufficient to accelerate the denaturation of the hemoglobin and includes at least one metal ion selected from the group consisting of sodium (Na), potassium (K), magnesium (Mg) and calcium (Ca).

An analysis device for use in measuring hemoglobin and hemoglobin A1c as a hemoglobin derivative in a sample containing a blood component including red blood cells, comprising:

• a sample addition site for adding the sample,
• a denaturation section connected to the sample addition site, for denaturing hemoglobin in the added sample with a reagent composition containing at least a nonionic surfactant, an oxidizing agent and a metal salt, wherein the denaturation section comprises the reagent composition;
• a hemoglobin detection section connected to the denaturation section, for detecting the denatured hemoglobin;
• an immunoassay section retaining an antibody specifically binding to a denatured site of hemoglobin A1c as a hemoglobin derivative denatured, and
• a hemoglobin derivative detection section for detecting the denatured hemoglobin A1c as a hemoglobin derivative after the hemoglobin A1c as a hemoglobin derivative is denatured by the reagent composition, the hemoglobin derivative detection section performing an immunoassay using the antibody for the detection,
• wherein the metal salt included in the reagent composition is present in an amount sufficient to accelerate the denaturation of the hemoglobin and includes at least one metal ion selected from the group consisting of sodium (Na), potassium (K), magnesium (Mg) and calcium (Ca),
• wherein the denaturation section, the hemoglobin detection section, the immunoassay section and the hemoglobin derivative detection section are in fluid communication with one another.