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US 8,574,905 B2Granted
Hepatocyte lineage cells
AssigneeViaCyte, Inc.
InventorsKevin D'Amour, Emmanuel E. Baetge
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Abstract
Disclosed herein are methods for producing liver precursor cells as well as hepatocyte cells form pluripotent and/or multipotent cells. Also disclosed herein are methods of enriching isolating and/or purifying liver precursor cells and/or hepatocyte cells. Further disclosed are compositions comprising cell cultures and cell populations that are enriched for liver precursor cells or hepatocyte cells.
Claims1
A method of enriching a population of liver precursor cells, said method comprising:
- providing a cell population comprising human pluripotent cells with a growth factor of the TGFβ superfamily;
- allowing sufficient time for at least a portion of said human pluripotent cell population to differentiate into definitive endoderm cells;
- providing said cell population comprising definitive endoderm cells with BMP4 and FGF10;
- allowing sufficient time for at least a portion of said definitive endoderm cells to differentiate into liver precursor cells;
- contacting said cell population comprising liver precursor cells with a reagent that binds to a marker selected from the group consisting of DPP4, NOSTRIN, DOK4, SLC35D1, OCDH17, FN1, HLA-C, HLA-B, CYP4X1, CPHA6, PP1057 HPX and DGKB;
- and
- separating cells bound to said reagent from cells that are not bound to said reagent, thereby producing an enriched population of liver precursor cells.
4 more elements
Dependencies are inferred from the claim text.