Nucleic acid cleavage complex and method for using the same
AssigneeNational Cheng Kung University
InventorsDar-Bin Shieh, Ying-Ying Li, Hung-Ching Lu
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Abstract
A nucleic acid cleavage complex is disclosed, which includes: a nanoparticle, a nucleic acid cleavage reagent, and a polynucleotide chain specifically recognizing a sequence of a target nucleic acid and having a first terminal and a second terminal opposite to the first terminal, wherein the first terminal is connected to the nanoparticle, the second terminal is connected to the nucleic acid cleavage reagent, and the first terminal sequence and the second terminal sequence are base-paired to make the polynucleotide chain form a hairpin. Also, a method for using the nucleic acid cleavage complex is also disclosed.
Claims14
A nucleic acid cleavage complex, comprising:
- a nanoparticle;
- a nucleic acid cleavage reagent, wherein said nucleic acid cleavage reagent is photo-excitable by visible light, ultraviolet light, and near infrared;
- and
- a polynucleotide chain which specifically recognizes a sequence of a target nucleic acid, and has a first terminal sequence and a second terminal sequence opposite to the first terminal sequence, wherein the first terminal sequence is connected to the nanoparticle, the second terminal sequence is connected to the nucleic acid cleavage reagent, and the first terminal sequence and the second terminal sequence are base-paired to make the polynucleotide chain form a hairpin.
A method of using a nucleic acid cleavage reagent, comprising the following steps:
- (a) providing a target nucleic acid and a nucleic acid cleavage complex, wherein said nucleic acid cleavage complex comprises:
- a nanoparticle;
- a nucleic acid cleavage reagent, wherein said nucleic acid cleavage reagent is photo-exiteable by visible light, ultraviolet light, and near infrared;
- and
- a polynucleotide chain which has a first terminal sequence and a second terminal sequence opposite to the first terminal sequence, wherein the first terminal sequence is connected to the nanoparticle, the second terminal sequence is connected to the nucleic acid cleavage reagent, and the first terminal sequence and the second terminal sequence are base-paired to make the polynucleotide chain form a hairpin;
- (b) allowing the nucleic acid cleavage complex to contact the target nucleic acid, wherein the polynucleotide chain of the nucleic acid cleavage complex specifically recognizes the sequence of the target nucleic acid;
- and
- (c) activating the nucleic acid cleavage reagent of the nucleic acid cleavage complex to cleave the sequence of the target nucleic acid.
Dependencies are inferred from the claim text.