Fatty alcohol forming acyl reductases (FARs) and methods of use thereof
OwnerBehnaz Behrouzian, Louis Clark +3inventors
InventorsBehnaz Behrouzian, Louis Clark, Yihui Zhu, Michael Clay, Kristian Karlshoej
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Abstract
The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same.
Claims21
A host cell comprising a recombinant polynucleotide sequence encoding a fatty alcohol forming acyl-CoA reductase (FAR) variant that has at least 90% sequence identity to SEQ ID NO:2, wherein the host cell is a bacterium and wherein the variant comprises a substitution at one or more positions selected from position 134, position 138, position 511, position 510, position 2, position 140, position 421, and position 458, wherein the position is numbered with reference to SEQ ID NO:2, with the proviso the FAR variant does not have the sequence of SEQ ID NO:5.
An E. coli cell comprising a recombinant polynucleotide sequence encoding a fatty alcohol forming acyl-CoA reductase (FAR) variant that has at least 90% sequence identity to SEQ ID NO:2, wherein the variant comprises a substitution at one or both of position 138 and position 511, wherein the positions are numbered with reference to SEQ ID NO:2.
Dependencies are inferred from the claim text.