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US 8,574,877 B2Granted
Production of fatty alcohols with fatty alcohol forming acyl-CoA reductases (FAR)
AssigneeCodexis, Inc.
InventorsRobert McDaniel, Behnaz Behrouzian, Louis Clark, Douglas A. Hattendorf, Fernando Valle
Life of this patent
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Abstract
The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
Claims21
A method of producing a fatty alcohol composition comprising:
- a) culturing a recombinant microorganism in a suitable culture medium, wherein the recombinant microorganism comprises a gene encoding a heterologous fatty acyl reductase (FAR) enzyme having at least 75% sequence identity to SEQ ID NO:
- 6, and
- b) allowing expression of said gene, wherein said expression results in the production of a composition of fatty alcohols.
Dependencies are inferred from the claim text.