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US 8,574,845 B2Granted
Method for identifying an increased susceptibility to ulcerative colitis
AssigneeChristian-Albrechts-Universität zu Kiel
InventorsStefan Schreiber, Andre Franke
Life of this patent
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Abstract
A method is provided for detecting an increased susceptibility to ulcerative colitis by determining the presence of a polymorphism at position 256 of the nucleotide sequence of rs3024505 in which C is substituted by T or the presence of a polymorphism at position 501 of the nucleotide sequence of rs12612347 in which G is substituted by A.
Claims4
A method of identifying individuals having a nucleic acid molecule, comprising:
- (a) amplifying a DNA fragment comprising an individual's nucleotide sequence selected from the group of:
- (i) nucleic acid molecules comprising the nucleotide sequence as depicted in SEQ ID NO:
- 2;
- and
- (ii) nucleic acid molecules the complementary strand of which hybridizes to a nucleic acid molecule of (i),
- using an oligonucleotide primer which specifically hybridizes to sequences within the individual's nucleotide sequence;
- (b) sequencing said amplified DNA fragment with dideoxy sequencing;
- (c) repeating steps (a) and (b) until the individual's nucleotide sequence is completely sequenced;
- (d) comparing the individual's nucleotide sequence with the nucleotide sequence as depicted in SEQ ID NO:
- 1;
- (e) determining the presence of a polymorphic variation in the individual's nucleotide sequence at position 256 of the nucleotide sequence as depicted in SEQ ID NO:
- 4.
9 more elements
A method of identifying individuals having a nucleic acid molecule, comprising:
- (a) amplifying a DNA fragment comprising an individual's nucleotide sequence selected from the group of:
- (i) nucleic acid molecules comprising the nucleotide sequence as depicted in SEQ ID NO:
- 4;
- and
- (ii) nucleic acid molecules the complementary strand of which hybridizes to a nucleic acid molecule of (i),
- using an oligonucleotide primer which specifically hybridizes to sequences within the individual's nucleotide sequence;
- (b) sequencing said amplified DNA fragment with dideoxy sequencing;
- (c) repeating steps (a) and (b) until the individual's nucleotide sequence is completely sequenced;
- (d) comparing the individual's nucleotide sequence with the nucleotide sequence as depicted in SEQ ID NO:
- 3;
- (e) determining the presence of a polymorphic variation in the individual's nucleotide sequence at position 501 of the nucleotide sequence as depicted in SEQ ID NO:
- 4.
9 more elements
Dependencies are inferred from the claim text.