US 8,574,844 B2 · Quantitative real-time assay for Noroviruses and Enteroviruses with built in quality control standard

Abstract

A method is provided for reverse transcription-polymerase chain reaction (RT-PCR) accomplished by: a) amplifying a reverse transcribed cDNA in a mixture containing Norovirus Genogroup I and Norovirus Genogroup II primers and probes, in which the Norovirus primers and probes can distinguish between Genogroup I and Genogroup II viruses; b) quantifying virus; and c) normalizing data based on a universal internal RNA control. Optionally, the method may also include primers and probes for Enteroviruses. The present invention also provides a reaction mixture containing Norovirus Genogroup I and Norovirus Genogroup II primers and probes, in which the Norovirus primers and probes can distinguish between Genogroup I and Genogroup II viruses and universal internal RNA control primers and probes.

Claims32

Dependent claims

A reverse transcription polymerase chain reaction (RT-PCR) mixture comprising:

a) an oligonucleotide probe and primers specific for Norovirus Genogroup I;
b) an oligonucleotide probe and primers specific for Norovirus Genogroup II;
c) a universal internal nucleic acid molecule, wherein said molecule contains at least one forward primer annealing site, at least one reverse primer annealing site, and at least one amplifiable region;
d) a universal internal control oligonucleotide probe;
and
e) a universal internal RNA control primer,
wherein i) the universal internal control oligonucleotide probe comprises SEQ ID NO:13;
or
ii) the universal internal RNA control primer is selected from the group consisting of SEQ ID NO:11, SEQ ID NO:12 and combination thereof;
or
iii) both (i) and (ii).

8 more elements

Dependencies are inferred from the claim text.

Quantitative real-time assay for Noroviruses and Enteroviruses with built in quality control standard

Life of this patent

Quantitative real-time assay for Noroviruses and Enteroviruses with built in quality control standard

Life of this patent

Abstract

Claims32