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US 8,574,838 B2Granted
Methods and kits for miRNA isolation and quantitation
AssigneeUniversity of Medicine and Dentistry of New Jersey
InventorsChunxiang Zhang, Yunhui Cheng, Xiaojun Liu, Jian Yang
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Abstract
The present invention is a kit and method for isolating and quantitating miRNA and to the use of such methods in the diagnosis and prognosis of disease.
Claims5
A kit for isolating and analyzing miRNA from a biological fluid comprising
- (a) a denaturing solution containing 20-25 mM sodium citrate and glycogen;
- (b) extraction reagents;
- (c) reverse transcriptase (RT) and an RT primer of SEQ ID NO:1;
- (d) reagents for real time PCR;
- and
- (e) mature miRNA.
3 more elements
A method for diagnosing or prognosing an acute myocardial infarction comprising
- (a) providing a blood or plasma sample from a subject;
- (b) quantitating the amount of miR-1 in the subject's sample using a primer of SEQ ID NO:1;
- and
- c) comparing the amount of miR-1 in the subject's sample with the amount of miR-1 in a reference sample, wherein an increase in the amount of miR-1 in the subject's sample compared with the reference sample is indicative of an acute myocardial infarction diagnosis or prognosis.
1 more element
Dependencies are inferred from the claim text.