Methods and compositions for amplifying a detectable signal
AssigneeGreat Basin Scientific
InventorsRobert D. Jenison, Joshua Klonoski, Anthony R. Torres
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Abstract
Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.
Claims49
A method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising:
- providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups;
- binding a detectable label complex to one or more of the amine groups on the amplification polymer;
- reacting amine groups not bound to a detectable label complex with an acetylating compound to create an amide group;
- wherein the complex is neutrally charged and water soluble.
A complex for amplifying a signal in a diagnostic assay for a nucleic acid analyte, comprising:
- an amplification polymer bound to a nucleic acid analyte,
- wherein the amplification polymer comprises a plurality of detectable label complexes bound to amine groups and a plurality of amide groups derived from a reaction of an amine group with an acetylating compound,
- and wherein the complex is neutrally charged and water soluble.
A kit for amplifying a signal in a diagnostic assay for a nucleic acid analyte, comprising:
- a capture molecule that specifically binds the nucleic acid analyte;
- an amplification polymer adapted to be conjugated to the nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups;
- a conjugation compound capable of conjugating the amplification polymer to the nucleic acid analyte;
- an acetylating compound capable of reaction with amine groups on the amplification polymer to create amide groups;
- and
- a detectable label complex.
Dependencies are inferred from the claim text.