US 8,574,831 B2 · Mutational profiles in HIV-1 reverse transcriptase correlated with phenotypic drug resistance

Abstract

The invention provides novel mutations, mutation combinations or mutational profiles of HIV-1 reverse transcriptase and/or protease genes correlated with phenotypic resistance to HIV drugs. More particularly, the present invention relates to the use of genotypic characterization of a target population of HIV and the subsequent correlation of this information to phenotypic interpretation in order to correlate virus mutational profiles with drug resistance. The invention also relates to methods of utilizing the mutational profiles of the invention in databases, drug development, i.e., drug design, and drug modification, therapy and treatment design, clinical management and diagnostic analysis.

Claims2

Dependent claims

A method of evaluating the effectiveness of a protease inhibitor as an antiviral therapy of an HIV-infected patient, comprising:

(a) collecting a sample from an HIV-infected patient;
(b) amplifying viral genomic sequences using primers targeting the HIV protease gene and determining whether the sample comprises a nucleic acid encoding a HIV protease having the mutation I84C combined with at least one additional mutation selected from L10I, K20R, K20T, L24I, L33F, L33I, M36I, M46L, A71T, A71V, V77I, V82I, or L90M, wherein the codon position number is compared to the sequence of reference wild-type HIV IIIB/LAI;
and
(c) using the presence of said at least one mutations of step b) to evaluate the effectiveness of said antiviral therapy.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

11/933,747Filed 1 Nov 2007 · continuation

Priority

20 Oct 2000

Published

US 2008/0286754 A120 Nov 2008

Granted

5 Nov 2013

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

G16B 20/20
ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations: allele or variant detection, e.g. single…
G16B 20/00
Ict specially adapted for functional genomics or proteomics
G16B 20/50
ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations: mutagenesis
G16B 50/00
Ict programming tools or database systems specially adapted for bioinformatics

Family

Application60/241,801Us provisional application · filed 20 Oct 2000

Application60/241,844Us provisional application · filed 20 Oct 2000

PCT/EP01/12338Parent of this continuation · filed 22 Oct 2001

US 2008/0286754 A1Pre-grant publication · published 20 Nov 2008

Application10/399,920Parent of this continuation

US 2004/0073378 A1Published application

US 8,574,831 B2This patent

From the US filing history. Foreign family members aren’t listed.

Mutational profiles in HIV-1 reverse transcriptase correlated with phenotypic drug resistance

Life of this patent

Mutational profiles in HIV-1 reverse transcriptase correlated with phenotypic drug resistance

Life of this patent

Abstract

Claims2