Process for demineralization of bone matrix with preservation of natural growth factors

A method for producing a demineralized cancellous bone matrix, the method comprising:

• placing a cancellous bone body in a first processing solution comprising an acid at a pH of greater than about pH=0 to demineralize the cancellous bone body;
• periodically removing the cancellous bone body from the first processing solution at specific time intervals to perform a compression test on the cancellous bone body, wherein the compression test comprises applying a compressive force in a range from about 10 g-force/cm2 to about 4000 g-force/cm2;
• and
• after the cancellous bone body is uniformly compressed to less than about 60 percent of its original shape, exposing the cancellous bone body to a second processing solution.