Hatching kit for toxicity test

A kit for conducting a toxicity test, comprising:

• a first container including a plurality of embryos of a species in a diapause III state and a storage medium that maintains said embryos in a diapause III state, controls moisture content, and keeps the individual embryos separated;
• a plurality of second containers, each second container comprising 1) a first conical closed end configured for receiving a plurality of said embryos in a diapause III state and a hatching medium for allowing said embryos to hatch into hatchlings and 2) a second open end having a beveled edge for contacting with 3 material to be tested for toxicity, wherein said second container facilitates embryo contact to induce or promote hatching;
• and a third container comprising a plurality of holding cells configured to hold the plurality of second containers at an angle such that the plurality of holding cells holds the plurality of second containers at a 45 degree angle, thereby allowing hatchlings to swim free from the plurality of second containers into the plurality of holding cells.