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US 8,574,599 B1Granted
Methods and products for inducing mucosal immunity
AssigneeOttawa Hospital Research Institute
InventorsMichael J. McCluskie, Heather L. Davis
Life of this patent
Estimating the term…
Abstract
The invention relates methods and products for inducing mucosal immunity. In particular, the invention relates to the use of immunostimulatory oligonucleotides containing a CpG motif for inducing mucosal immunity. The CpG immunostimulatory oligonucleotides may be administered alone or in combination with antigen and/or with other adjuvants.
Claims52
A method for inducing a mucosal immune response, comprising:
- administering to a subject in need of a mucosal immune response an effective amount for inducing a mucosal immune response of an oligonucleotide 8 to 100 nucleotides in length, having a sequence including at least the following formula:
- 5′X1X2CGX3X4 3′
- wherein C is unmethylated, wherein X1, X2, X3, and X4 are nucleotides, and
- an antigen,
- wherein the antigen is not encoded in a nucleic acid vector, the oligonucleotide and the antigen are both administered vaginally, rectally, intranasally, ocularly, or by inhalation to the subject, a cytokine and an immune stimulating complex are not administered to the subject, and the antigen is not a Streptococcus pneumoniae antigen.
2 more elements
A method for inducing a mucosal immune response, comprising:
- administering to a subject in need of a mucosal immune response an effective amount for inducing a mucosal immune response of an oligonucleotide 8 to 100 nucleotides in length, having a sequence including at least the following formula:
- 5′X1X2CGX3X4 3′
- wherein C is unmethylated, wherein X1, X2, X3, and X4 are nucleotides,
- a non-oligonucleotide mucosal adjuvant that is not an immune stimulating complex, and
- an antigen,
- wherein the antigen is not encoded in a nucleic acid vector, and wherein the oligonucleotide, the antigen, and the non-oligonucleotide mucosal adjuvant are all administered rectally, intravaginally, or ocularly, to the subject, and a cytokine is not administered to the subject.
3 more elements
A method for inducing a mucosal immune response, comprising:
- administering to a subject in need of a mucosal immune response an effective amount for inducing a mucosal immune response of an oligonucleotide 8 to 100 nucleotides in length, having a sequence including at least the following formula:
- 5′X1X2CGX3X4 3′
- wherein C is unmethylated, wherein X1, X2, X3, and X4 are nucleotides, and
- a viral antigen,
- wherein the antigen is not encoded in a nucleic acid vector, the oligonucleotide and the antigen are both administered vaginally, rectally, intranasally, ocularly, or by inhalation to the subject, and a cytokine and an immune stimulating complex are not administered to the subject.
2 more elements
A method for inducing a mucosal immune response, comprising:
- administering to a subject in need of a mucosal immune response an effective amount for inducing a mucosal immune response of an oligonucleotide 8 to 100 nucleotides in length, having a sequence including at least the following formula:
- 5′X1X2CGX3X4 3′
- wherein C is unmethylated, wherein X1, X2, X3, and X4 are nucleotides, and
- passively exposing the subject to an antigen,
- wherein the antigen is not encoded in a nucleic acid vector, oligonucleotide administration and antigen exposure both occur vaginally, rectally, intranasally, or by inhalation, and a cytokine and an immune stimulating complex are not administered to the subject.
2 more elements
A method for inducing a mucosal immune response, comprising:
- administering to a subject in need of a mucosal immune response an effective amount for inducing a mucosal immune response of an oligonucleotide 8 to 100 nucleotides in length, having a sequence including at least the following formula:
- 5′X1X2CGX3X4 3′
- wherein C is unmethylated, wherein X1, X2, X3, and X4 are nucleotides, and
- an antigen,
- wherein the antigen is not encoded in a nucleic acid vector, the oligonucleotide and the antigen are both administered vaginally, rectally, or ocularly to the subject, and a cytokine and an immune stimulating complex are not administered to the subject.
2 more elements
A method for inducing a mucosal immune response, comprising:
- administering to a subject in need of a mucosal immune response an effective amount for inducing a mucosal immune response of an oligonucleotide 8 to 100 nucleotides in length, having a sequence including at least the following formula:
- 5′X1X2CGX3X4 3′
- wherein C is unmethylated, wherein X1, X2, X3, and X4 are nucleotides, and
- an antigen,
- wherein the antigen is not encoded in a nucleic acid vector and is not a Streptococcus pneumoniae antigen, the oligonucleotide and the antigen are both administered intranasally or by inhalation to the subject, and a cytokine and an immune stimulating complex are not administered to the subject.
2 more elements
A method for inducing a mucosal immune response, comprising
- mucosally administering to a subject an oligonucleotide 8 to 100 nucleotides in length and comprising 5′X1X2CGX3X4 3′ wherein C is unmethylated and X1, X2, X3, and X4 are nucleotides, and an antigen that is not encoded in a nucleic acid vector, and
- systemically administering to the subject the antigen.
A method for inducing an immune response, comprising
- systemically administering to a subject, as a priming dose, an antigen that is not encoded in a nucleic acid vector, and
- mucosally administering to the subject, as a boost dose, an oligonucleotide 8-100 nucleotides in length and comprising 5′X1X2CGX3X4 3′ wherein C is unmethylated and X1, X2, X3, and X4 are nucleotides, and the antigen.
A method for inducing a mucosal immune response in a subject, comprising
- mucosally administering to a subject, as a boost dose, an oligonucleotide 8-100 nucleotides in length and comprising 5′X1X2CGX3X4 3′ wherein C is unmethylated and X1, X2, X3, and X4 are nucleotides, and an antigen that is not encoded by a nucleic acid vector, wherein the subject has received a priming dose of antigen administered systemically.
Dependencies are inferred from the claim text.