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US 8,574,593 B2Granted
Influenza hemagglutinin and neuraminidase variants
AssigneeMedImmune, LLC
InventorsChin-Fen Yang, George Kemble, Chongguang Liu
Life of this patent
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Abstract
Polypeptides, polynucleotides, methods, compositions, and vaccines comprising influenza hemagglutinin and neuraminidase variants are provided.
Claims20
A 6:2 reassortant influenza B virus, wherein said virus comprises 6 internal genome segments from one or more donor viruses and two surface antigen genome segments, wherein the surface antigen genome segments encode an HA and a NA polypeptide, and wherein the HA polypeptide comprises the amino acid sequence of SEQ ID NO:
- 93.
A method for producing an influenza B virus in cell culture, the method comprising:
- i) introducing into a population of host cells, which population of host cells is capable of supporting replication of influenza virus, a plurality of vectors comprising nucleotide sequences corresponding to:
- (a) at least 6 internal genome segments of one or more donor viruses, and at least one genome segment encoding an HA surface antigen polypeptide, wherein the surface antigen polypeptide comprises the amino acid sequence from SEQ ID NO:
- 93;
- or
- (b) at least 6 internal genome segments of one or more donor viruses, which one or more donor viruses comprises one or more phenotypic attributes selected from the group consisting of:
- attenuated, cold adapted and temperature sensitive;
- and at least one genome segment encoding an HA surface antigen, wherein the surface antigen polypeptide comprises the amino acid sequence of SEQ ID NO:
- 93;
- ii) culturing the population of host cells at a temperature less than or equal to 35° C.;
- and,
- iii) recovering influenza virus.
8 more elements
Dependencies are inferred from the claim text.