Monoclonal antibodies specific to hemagglutinin and neuraminidase from influenza virus H5-subtype or N1-subtype and uses thereof
AssigneeTemasek Life Sciences Laboratory Limited
InventorsHong Liang Qian, Fang He, Hwei-Sing Kwang
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Abstract
Monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes or neuraminidase glycoprotein of N1 subtypes of avian influenza virus (AIV) are provided. The monoclonal antibodies and related binding proteins are useful for the detection of H5 and N1 subtypes of AIV, including H5N1 subtypes and provide means for the diagnosis, surveillance and treatment of dangerous viral infections.
Claims24
An isolated binding protein that binds specifically to a conformational epitope of the hemagglutinin envelope glycoprotein of an H5 subtype of avian influenza virus and that has the immunological binding characteristics of monoclonal antibody 4F8 as produced by hybridoma 4F8 which is deposited with the American Type Culture Collection with Accession No. PTA-9397, wherein the conformational epitope includes amino acid 205 (Lys).
Monoclonal antibody 4F8 as produced by hybridoma 4F8 which is deposited with the American Type Culture Collection with Accession Number PTA-9397.
A method for detecting H5 subtype avian influenza virus in a biological specimen which comprises contacting the specimen with a binding protein that binds specifically to a conformational epitope of the hemagglutinin envelope glycoprotein of an H5 subtype of avian influenza virus said binding protein having the immunological binding characteristics of monoclonal antibody 4F8 as produced by hybridoma 4F8 which is deposited with the American Type Culture Collection with Accession No. PTA-9397, wherein the conformational epitope includes amino acid 205 (Lys).
A kit for detecting H5 subtype avian influenza virus in a biological specimen which comprises a binding protein that binds specifically to a conformational epitope of the hemagglutinin envelope glycoprotein of an H5 subtype of avian influenza virus, said binding protein having the immunological binding characteristics of monoclonal antibody 4F8 as produced by hybridoma 4F8 which is deposited with the American Type Culture Collection with Accession No. PTA-9397, wherein the conformational epitope includes amino acid 205 (Lys), together with at least one reagent for the detection of binding of said binding protein to said envelope glycoprotein.
A method of treating a subject infected with H5 subtype avian influenza virus which comprises administering to said subject an effective amount of a binding protein that binds specifically to a conformational epitope of the hemagglutinin envelope glycoprotein of an H5 subtype of avian influenza virus that has the immunological binding characteristics of monoclonal antibody 4F8 as produced by hybridoma 4F8 which is deposited with the American Type Culture Collection with Accession No. PTA-9397, wherein the conformational epitope includes amino acid 205 (Lys).
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