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US 8,574,580 B2Granted
Monoclonal antibodies that recognize neurospheres or neural progenitor cells
AssigneeConsejo Superior de Investigaciones Cientificas
InventorsAugusto Silva Gonzalez, Ignacio Del Valle Torres, Leyre García Benzaquen
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Abstract
Method for generating monoclonal antibodies that recognize progenitor cells. Said method comprises immunization of an Armenian hamster with neurospheres obtained from olfactory bulb cells from a 13.5-day mouse embryo and subsequent selection of the antibodies by means of neurosphere flow cytometry in the presence of propidium iodide. The antibodies thus obtained may be of use in the enrichment of cell cultures in progenitor cells, primarily neural progenitor cells.
Claims3
A monoclonal antibody selected from among:
- a. a monoclonal antibody, NILO1, produced by the hybridoma deposited under the DSM access number No. ACC2887 in the international deposit authority Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), Braunschweig, Germany;
- and
- b. a monoclonal antibody, NILO2, produced by the hybridoma deposited under the DSM access number No. ACC2881 in the international deposit authority Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), Braunschweig, Germany;
- wherein the monoclonal antibody binds neurospheres that are positive for nestin, GFAP, and Ki67 or neural progenitor cells that are positive for doublecortin (DCX), Ki67, GFAP, Vimentin and beta III tubulin.
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Dependencies are inferred from the claim text.