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US 8,574,567 B2Granted
Multipotent stem cells and uses thereof
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsKeith D. Crawford, Chris Southgate
Life of this patent
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AssigneeThe Brigham and Women's Hospital, Inc.
InventorsKeith D. Crawford, Chris Southgate
Estimating the term…
The invention provides a quiescent stem cell having the capacity to differentiate into ectoderm, mesoderm and endoderm, and which does not express cell surface markers including MHC class I, MHC class II, CD44, CD45, CD13, CD34, CD49c, CD73, CD105 CD90, CD66A, CD66E, CXCR4, CD133 or an SSEA. The invention further provides a proliferative stem cell, which expresses genes including Oct-4, Nanog, Sox2, GDF3, P16INK4, BMI, Notch, HDAC4, TERT, Rex-1, TWIST, KLF-4 and Stella but does not express cell surface markers including MHC class I, MHC class II, CD44, CD45, CD13, CD34, CD49c, CD73, CD105, CD90, CD66A, CD66E, CXCR4, CD133 or an SSEA. The cells of the invention can be isolated from adult mammals, have embryonic cell characteristics, and can form embryoid bodies. Methods for obtaining the stem cells, as well as methods of treating diseases and differentiated the stem cells, are also provided.
An isolated human adult stem cell that is capable of proliferating and differentiating into at least two of ectoderm, mesoderm, or endoderm, expresses at least one of Oct-4, KLF-4, Nanog, Sox-2, Rex-1, GDF-3, and Stella, and does not detectibly express CD13, CD45, CD90, and CD34 and further does not detectibly express at least one of MHC class I, MHC class II, CD44, CD49c, CD73, CD66A, CD66E, CXCR4, CD133 or an SSEA.
An isolated quiescent human adult stem cell that is capable of proliferating and differentiating into at least two of ectoderm, mesoderm, and endoderm and does not detectibly express Oct-4, CD13, CD45, CD90, CD34 and further does not detectibly express at least one of MHC class I, MHC class II, CD44, CD49c, CD73, CD66A, CD66E, CXCR4, CD133 or an SSEA.
A population of isolated human adult stem cells that are capable of proliferating and differentiating into at least two of ectoderm, mesoderm, and endoderm, express at least one of Oct-4, KLF-4, Nanog, Sox-2, Rex-1, GDF-3, and Stella, and does not detectibly express CD13, CD45, CD90, CD34 and further does not detectibly express at least one of MHC class I, MHC class II, CD44, CD49c, CD73, CD66A, CD66E, CXCR4, CD133 or an SSEA, wherein from about 10% to about 30% of the population of human adult stem cells are quiescent.
Dependencies are inferred from the claim text.