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US 8,574,494 B2Granted
In situ heat induced antigen recovery and staining method
OwnerLee Angrosinventor
Life of this patent
Estimating the term…
Abstract
An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. The reaction conditions for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide.
Claims28
A method comprising:
- disposing a microscope slide on at least one slide support element of an automated apparatus comprising a plurality of slide support elements, each of which is sized to support a single microscope slide, and wherein each slide support element can be moved independently of each other slide support element and wherein said independent movement of the slide support element is controlled automatically, and the apparatus further comprising a separate heating element incorporated in each slide support element for independently heating a microscope slide disposed on the slide support element such that when each slide support element is moved, the separate heating element therein is moved independently of each other separate heating element;
- treating the microscope slide disposed upon the at least one slide support element forming a treated microscope slide;
- and
- independently moving the at least one slide support element having the treated microscope slide thereon.
1 more element
A method comprising:
- disposing a first microscope slide on a first slide support element and a second microscope slide on at least one second slide support element of an automated apparatus wherein the first slide support element is sized to support a single microscope slide and the at least one second slide support element is sized to support a single microscope slide, and wherein the first slide support element and the at least one second slide support element can be moved independently of each other, the apparatus further comprising a first heating element for independently heating the first microscope slide disposed on the first slide support element and a second heating element for independently heating the second microscope slide disposed on the at least one second slide support element;
- and
- independently moving the first slide support element having the first microscope slide thereon from a position for placement of the microscope slide thereon to a first treatment position while the at least one second slide support element is in a second treatment position for treating the second microscope slide thereon and wherein said movement of the first slide support element and the at least one second slide support element is controlled automatically.
A method comprising:
- disposing a first microscope slide on a first slide support element and a second microscope slide on at least one second slide support element of an automated apparatus wherein the first slide support element is sized to support a single microscope slide and the at least one second slide support element is sized to support a single microscope slide, and wherein the first slide support element and the at least one second slide support element can be moved independently of each other, the apparatus further comprising a first heating element for independently heating the first microscope slide disposed on the first slide support element and a second heating element for independently heating the second microscope slide disposed on the at least one second slide support element;
- and
- independently moving the first slide support element having the first microscope slide thereon from a first treatment position to a position for removal of the first microscope slide thereon while the at least one second slide support element is in a second treatment position for treating the second microscope slide thereon and wherein said movement of the first slide support element and the at least one second slide support element is controlled automatically.
A method comprising:
- disposing a microscope slide on at least one slide support element of an automated apparatus comprising a slide support assembly comprising a plurality of slide support elements, each of which is sized to support a single microscope slide thereon, and wherein the slide support elements are automatically movable independently of each other slide support element to positions for placement of or removal of the microscope slides thereon and to treatment positions for treating the microscope slides thereon, and wherein each slide support element has a separate heating element incorporated therein for independently heating said microscope slide disposed on the slide support element such that when each slide support element is moved, the separate heating element therein is moved independently of each other separate heating element, and wherein each separate heating element of the plurality of slide support elements is separately controllable by a microprocessor;
- moving the at least one slide support element from the corresponding position for placement or removal of a microscope slide to the corresponding treatment position for treating the microscope slide disposed thereon.
A method comprising:
- disposing a microscope slide on at least one slide support element of an automated apparatus comprising a slide support assembly comprising a plurality of slide support elements, each of which is sized to support a single microscope slide thereon, and wherein the slide support elements are automatically movable independently of each other slide support element to positions for placement of or removal of the microscope slides thereon and to treatment positions for treating the microscope slides thereon, and wherein each slide support element has a separate heating element incorporated therein for independently heating said microscope slide disposed on the slide support element such that when each slide support element is moved, the separate heating element therein is moved independently of each other separate heating element, and wherein each separate heating element of the plurality of slide support elements is separately controllable by a microprocessor;
- moving the at least one slide support element from the corresponding treatment position to the corresponding position for placement or removal of the microscope slide thereon.
A method comprising:
- disposing a microscope slide on at least one slide support element of an automated apparatus comprising a plurality of slide support elements, each of which is sized to support a single microscope slide, and wherein each slide support element can be moved independently of each other slide support element and wherein said independent movement of the slide support element is controlled automatically, and the apparatus further comprising a separate heating element incorporated in each slide support element for independently heating a microscope slide disposed on the slide support element such that when each slide support element is moved, the separate heating element therein is moved independently of each other separate heating element;
- treating the microscope slide disposed upon the at least one slide support element forming a treated microscope slide;
- and
- independently moving the at least one slide support element and the treated microscope slide thereon out of a treatment chamber to a removal position for removing the treated microscope slide from the at least one slide support element.
1 more element
A method comprising:
- disposing a microscope slide on at least one slide support element of an automated apparatus comprising a plurality of slide support elements, each of which is sized to support a single microscope slide, and wherein each slide support element can be moved independently of each other slide support element and wherein said independent movement of the slide support element is controlled automatically, and the apparatus further comprising a separate heating element incorporated in each slide support element for independently heating a microscope slide disposed on the slide support element such that when each slide support element is moved, the separate heating element therein is moved independently of each other separate heating element;
- independently moving the at least one slide support element having the microscope slide thereon from a placement position to a treatment position within a treatment chamber;
- and
- treating the microscope slide disposed upon the at least one slide support element in the treatment chamber.
1 more element
Dependencies are inferred from the claim text.