Method for production of chromatography media
AssigneeGE Healthcare Bio-Sciences AB
InventorsJan Bergstrom, Bo-Lennart Johansson
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Abstract
The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.
Claims11
A method for producing chromatography media comprising shell beads having an inner porous core and an outer shell, for chromatography techniques of biomolecules using immobilized buffering ligands in the core, comprising providing buffering ligands in the core of the beads, and providing binding ligands in the outer shell of the beads, wherein the buffering ligands and binding ligands are adjusted independently of each other.
Dependencies are inferred from the claim text.