Integrated chromatography devices and systems for monitoring analytes in real time and methods for manufacturing the same

A microfluidic system for sample analysis comprising:

• a reservoir assembly comprising at least two sample reservoirs;
• an integrated microfluidic chromatography device in fluid communication with the at least two sample reservoirs, the integrated microfluidic chromatography device comprising at least two separation columns integrated into a substrate and adapted to perform separate and simultaneous separations of samples, the separations being of different types, and at least one detector integrated into the substrate, at least one of the separation columns formed by depositing, using chemical vapor deposition (CVD), a polymer over neighboring etched regions of the substrate, one region being narrower and one region being wider, the depositing sufficient to line a portion of a bottom of and seal over the narrower etched region and define an inner channel and then coat an inner surface of the inner channel from the polymer diffusing through the wider region until the wider etched region is sealed over by the deposition;
• a pump adapted to deliver samples from the at least two sample reservoirs to the integrated microfluidic chromatography device;
• an analyte detection assembly comprising at least one detector in fluid communication with at least one separation column of the at least two separation columns;
• and
• a processing unit in electrical communication with the at least one detector.

An integrated microfluidic chromatography device comprising:

• a filter;
• and
• a separation column in fluid communication with the filter,
• wherein the filter and separation column were formed by depositing a polymer over an open etched channel of a substrate, the channel having regions, one etched region being narrower and another etched region being wider, the depositing sufficient to coat a portion of a bottom of and seal over the narrower etched region to define an inner channel for the filter and then coat an inner surface of the inner channel from an opening to the wider etched region until the wider etched region is sealed over to define the separation column.