Ultrathin multilayered films for controlled release of anionic reagents
AssigneeWisconsin Alumni Research Foundation
InventorsDavid M. Lynn, Jingtao Zhang, Xianghui Liu
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Abstract
Multilayered films, particularly ultrathin multilayered films comprising cationic polymers which are useful for controlled release of anionic species, particularly for controlled release of nucleic acids. The multilayer films herein are useful for temporal controlled released of anionic species, particularly one or more anionic peptides, proteins, nucleic acids or other anionic biological agents. In one aspect, the invention relates to multilayer films which release anionic species (anions) with separate and/or distinct release profiles, particularly wherein the anions are one or more anionic peptides, proteins or nucleic acids or other anionic biological agents.
Claims21
A method for controlling the release of one or more anions (as a function of time) from a film which comprises the steps of:
- (i) forming a polyelectrolyte assembly wherein the polyelectrolyte assembly comprises at least two anion/cationic polymer bilayers comprising one or more different anion/cationic polymer bilayers where at least a first bilayer is formed from a first cationic polymer and at least a second bilayer is formed from a second cationic polymer wherein the cationic polymers are selected from
- (a) a polymer of formula I:
- where:
- n+m+l+k+i=N, the total number of repeat units in the polymer;
- n, m, l, k and i are all integers;
- or
- m and l are integers, n is zero or an integer and i and k are zero;
- or
- m and l are integers, and n, i or k is zero or an integer;
- A1-3, B1-3, C1-3, D1-3 and E1-3 are linkers which may be the same or each may be different;
- Z1-Z5 are covalent bonds which may or may not be degradable bonds;
- R1-4 and R11-19, independently, can be hydrogen, alkyl groups, alkenyl groups, alkyny groups, cycloalkyl groups, heterocyclic groups, aryl groups, or heteroaryl groups, all of which groups are optionally substituted, with the exception that R1-4 are not esters;
- and
- R5-R10 are linkers or covalent bonds which may be the same or each may be different;
- or
- (b) a polymer of formula II:
- where n+m=N is the number of repeating units in the polymer;
- each y, independently, is 1, 2 or 3;
- each x, independently, is an integer ranging from 1-10;
- each R1, each R2, each R3 and each R4, independently, is selected from the group consisting of hydrogen, alkyl groups, alkenyl groups, alkynyl groups, carbocyclic groups, heterocyclic groups, aryl groups, heteroaryl groups, ether groups, all of which may be substituted or unsubstituted,
- wherein the first cationic polymer is selected from cationic polymers of formula I and the second cationic polymer is selected from cationic polymers of formula II,
- or
- wherein the first and second polymers are selected from cationic polymers of formula II and differ from each other in the value of (n)/N;
- and
- (ii) subjecting the polyelectrolyte assembly formed to conditions in which ester functions in the polymer of formula I or in the polymer of formula II are hydrolyzed to release the one or more anions.
A polyelectrolyte assembly which comprises a plurality of polyelectrolyte bilayers wherein a bilayer comprises one or more anions and one or more cationic polymers of formula:
- where n+m=N is the number of repeating units in the polymer;
- each y, independently, is 1, 2 or 3;
- each x, independently, is an integer ranging from 1-10;
- and
- each R1, each R2, each R3 and each R4, independently, is selected from the group consisting of hydrogen, alkyl groups, alkenyl groups, alkynyl groups, carbocyclic groups, heterocyclic groups, aryl groups, heteroaryl groups, ether groups, all of which may be substituted or unsubstituted.
Dependencies are inferred from the claim text.