# US 8,570,625 B2 · Device and method for generating an image of an object > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,570,625 B2 - Document ID: US8570625B2 - Status: Granted - Kind: B2 - Application number: 12/088,557 - Filed: 2006-09-14 - Published: 2008-10-16 - Granted: 2013-10-29 - Earliest claimed: 2005-09-29 - Assignees: Carl Zeiss MicroImaging GmbH - Applicants: Michael Kempe; Ralf Wolleschensky - Inventors: Michael Kempe; Ralf Wolleschensky - Web page: https://patentgrep.com/patent/US8570625B2 ## CPC - G02B 21/0032: {Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers (G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06)} - G02B 21/006: {focusing arrangements; selection of the plane to be imaged} - G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS ## Abstract A device for generating an image of an object is provided, comprising an illumination module, by means of which the object can be illuminated with a pattern whose phase is altered temporally, a recording module, by means of which a plurality of recordings of the object are carried out during the phase change of the pattern, and a processing module, which generates the image from the recordings, wherein the illumination module moves a light beam over the object and modulates its intensity synchronously with the movement such that the beam generates the pattern in scanning fashion. ## Claims ### Claim 1 A device for generating an image of an object, comprising an illumination module with which an object can be illuminated with a pattern, the phase of which is temporally changed; an exposure module with which several exposures of said object can be executed during the phase change of the pattern; and a processing module that produces the image from the exposures; wherein said illumination module moves a light beam over the object and focuses said light beam on the specimen in a punctiform or linear manner temporally modulates the intensity thereof synchronously with the movement thereby effecting spatial modulation of the intensity on the sample in such a way that the beam produces the pattern by means of scanning. ### Claim 2 (depends on 1) The device according to claim 1, wherein said illumination module focuses the beam on the specimen, particularly in a diffraction-limited manner. ### Claim 3 (depends on 1) The device according to claim 1, wherein said exposure module has a surface detector, which is used to detect, in a spatially resolved manner, synchronously with the movement of the beam, the specimen radiation produced by means of the beam. ### Claim 4 (depends on 3) The device according to claim 3, wherein said surface detector is a matrix or line detector. ### Claim 5 (depends on 1) The device according to claim 1, wherein said illumination module changes the phase in a temporally linear manner or periodic manner. ### Claim 6 (depends on 1) The device according to claim 1, wherein said illumination module has an optical switch for intensity modulation. ### Claim 7 (depends on 1) The device according to claim 1, further comprising a control module for controlling said illumination module, exposure module and processing module in such a way that illumination of the object occurs at different depths in the object by means of focusing the beam in the depths and, in each case, records several exposures in the different depths, such that the processing module can produce images from different depths of the object. ### Claim 8 (depends on 1) The device according to claim 1, wherein said illumination module produces several light beams, focuses on the object with these spaced from each other, and moves over the object and, in this connection, changes the intensity of the beams synchronously with the movement in such a way that the beams produce the pattern by means of scanning. ### Claim 9 A method for generating an image of an object comprising: illuminating an object with a periodic pattern, the phase of which is temporally changed, repeatedly exposing the object during the phase change, and generating an image from the exposures, wherein in the illumination step a light beam, the intensity of which is temporally modulated, is moved over the object, with the movement and intensity modulation of the light beam being synchronized thereby effecting spatial modulation of the intensity on the sample in such a way that the beam produces the pattern by means of scanning, and wherein said beam is focused in a punctiform or linear manner. ### Claim 10 (depends on 9) The method according to claim 9, wherein said beam is focused in a diffraction-limited manner. ### Claim 11 (depends on 9) The method according to claim 9, wherein in order to expose the object synchronously with the movement of the beam, the specimen radiation produced by means of the beam is detected in a spatially resolved manner. ### Claim 12 (depends on 9) The method according to claim 9, wherein the temporal change of the phase occurs in a linear or periodic manner. ### Claim 13 (depends on 9) The method according to claim 9, wherein in the different illumination steps a focusing of the light beam occurs in different depths of the specimen such that images can be produced from the different depths of said specimen. ### Claim 14 (depends on 9) The method according to claim 9, wherein several light beams, the intensities of which are temporally modulated, are focused, spaced apart from each other, on the object and are moved together over said object, with the movement and intensity modulation of the light beams being synchronized in such a way that the beams produce a pattern by means of scanning.