# US 8,570,500 B2 · Detector arrangement for a flow cytometry system

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- Number: US 8,570,500 B2
- Document ID: US8570500B2
- Status: Granted
- Kind: B2
- Application number: 12/860,790
- Filed: 2010-08-20
- Published: 2012-02-23
- Granted: 2013-10-29
- Earliest claimed: 2010-08-20
- Assignees: Stratedigm, Inc.
- Applicants: Shervin Javadi; Arjuna Karunaratne
- Inventors: Shervin Javadi; Arjuna Karunaratne
- Web page: https://patentgrep.com/patent/US8570500B2

## CPC

- G01N 15/1434: Optical arrangements
- G01N 15/1425: {using an analyser being characterised by its control arrangement}
- G01N 15/1459: {the analysis being performed on a sample stream}
- G01N 2015/1438: {Using two lasers in succession}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Systems and methods for regarding a cytometry system are disclosed. The system can include a plurality lasers which are spatially separated from each other. Each laser can be assigned to a single detector. The single detector can process multiple events from each laser by digital switching of signal processing circuitry. Additional detectors can be assigned to receive light in a similar manner.

## Claims

### Claim 1

A cytometry system comprising:
a computing system;
a plurality of laser sources controlled by the computing system to emit laser light, each laser source being spatially separated along a flow stream path;
a light condenser for collecting a plurality of light pulses that are separated by time of flight intervals, each light pulse being derived from the spatially separated laser light intersecting material flowing in the flow stream path; and
a first detector configured to receive the plurality of light pulses from the light condenser, the first detector being coupled to sampling circuitry,
wherein the computing system is configured to operate the sampling circuitry to process each light pulse according to the time of flight intervals,
wherein the computing system is configured to turn the sampling circuitry OFF during the time of flight intervals by actively discharging residual energy of one or more energy storage filter elements of the sampling circuitry,
wherein before each time of flight interval the residual energy is caused by filtering a prior light pulse, and wherein the residual energy caused by filtering the prior light pulse is actively discharged from the one or more energy storage filter element to prevent interference for processing a subsequent pulse by the sampling circuitry.

### Claim 2 (depends on 1)

The cytometry system of claim 1, wherein the laser sources emit different wavelengths of laser light with respect to each other.

### Claim 3 (depends on 2)

The cytometry system of claim 2, wherein at least one laser source comprises a plurality of collinear lasers.

### Claim 4 (depends on 1)

The cytometry system of claim 1, wherein the computing system is configured to turn the sampling circuitry ON at least partially during the time of flight intervals.

### Claim 5 (depends on 1)

The cytometry system of claim 1, wherein the computing system is configured to turn the sampling circuitry ON for pre-determined sampling intervals between the time of flight intervals and OFF during the time of flight intervals.

### Claim 6 (depends on 5)

The cytometry system of claim 5, wherein the time of flight intervals are determined from a flow rate of the material and the distance between the spatially separated laser sources.

### Claim 7 (depends on 5)

The cytometry system of claim 5, wherein the sampling circuitry is configured to only process a signal of each light pulse during the pre-determined sampling interval.

### Claim 8 (depends on 7)

The cytometry system of claim 7, wherein the sampling circuitry is configured to discharge the one or more energy storage filter element at some time point when the signal of the light pulse is not being sampled.

### Claim 9 (depends on 1)

The cytometry system of claim 1, further comprising:
a second detector configured to receive at least a portion of the plurality of light pulses from the light condenser, the second detector being coupled to its own sampling circuitry.

### Claim 10 (depends on 9)

The cytometry system of claim 9, wherein the computing system is configured to turn respective sampling circuitries ON between the time of flight intervals and OFF during the time of flight intervals.

### Claim 11 (depends on 10)

The cytometry system of claim 10, wherein the respective sampling circuitries are configured to discharge the one or more energy storage elements of the respective sampling circuitries at some time point when respective signals derived from the plurality of light pulses are not being sampled.

### Claim 12 (depends on 11)

The cytometry system of claim 11, wherein the respective sampling circuitries are configured to process each light pulse by triggering pulse width integrators, logarithmic and linear pulse peak detectors, and logarithmic and linear pulse area integrators of respective signal processing circuitries.

### Claim 13 (depends on 11)

The cytometry system of claim 11, wherein the respective signals are digitally converted before or after being processed by the respective signal processing circuitries.

### Claim 14

A method for operating a cytometry system, the method comprising:
receiving a first light pulse derived from a first laser interacting with a material;
triggering a first ON signal over a duration of a first predetermined sampling interval;
deriving pulse information from the first light pulse signal during the first ON signal via signal processing circuitry;
triggering an OFF signal to stop the signal processing circuitry from deriving pulse information from the first light pulse signal, the OFF signal corresponding to a time of flight interval of the material between the first laser and a spatially separated second laser;
receiving a second light pulse signal derived from the second laser interacting with the material;
triggering a second ON signal over a duration of a second predetermined sampling interval; and
deriving pulse information from the second light pulse signal during the second ON signal,
wherein residual energy of at least one energy storing filter element of the signal processing circuitry is actively discharged during the OFF signal,
wherein before each time of flight interval the residual energy is caused by filtering the first light pulse, and wherein the residual energy caused by filtering the first light pulse is actively discharged from the energy storage filter element to prevent interference for processing the second light pulse by the sampling circuitry.

### Claim 15 (depends on 14)

The method of claim 14, further comprising:
amplifying the first light pulse signal using a first amplifying circuit.

### Claim 16 (depends on 15)

The method of claim 15, further comprising:
amplifying the second light pulse signal using a second amplifying circuit.

### Claim 17 (depends on 14)

The method of claim 14, wherein at least one energy storing filter element of the signal processing circuitry is charged from the first light pulse signal during the first ON signal.

### Claim 18 (depends on 17)

The method of claim 17, wherein the at least one energy storage filter element comprises a capacitor, inductor, or a processor or computer readable medium including a digital implementation of a filter.

### Claim 19 (depends on 14)

The method of claim 14, wherein the at least one energy storing filter element of the signal processing circuitry is recharged from the second light pulse signal during the second ON signal.

### Claim 20 (depends on 14)

The method of claim 14, wherein the time of flight interval is derived from a flow rate of the material between the spatial separation of the first laser and the second laser.

### Claim 21 (depends on 14)

The method of claim 14, wherein deriving pulse information from the first and second light pulse signals respectively comprises triggering pulse width integrators, pulse peak detectors, and pulse area integrators of the signal processing circuitry.

### Claim 22 (depends on 14)

The method of claim 14, wherein the pulse information respectively derived from the first and second light pulse signals is converted to digital signals.